类视黄醇X受体基因调控动物季节性发情和发情周期的研究进展

doi:10.11843/j.issn.0366-6964.2019.08.001

Abstract

Abstract: Retinoid X receptors (RXRs) are members of the ligand-dependent nuclear receptor family. Many studies in the past have revealed the importance of RXRs in animal's seasonal estrus and estrous cycles, but RXRs must form dimers with themselves or other nuclear receptors to work. Different dimers play different roles, therefore their molecular mechanism of playing role in reproduction is difficult to study. Some studies have shown that RXRs expressed at different levels in the hypothalamic-pituitary-gonadal (HPG) axis tissues of seasonal estrus animals. Dimers of THRs/RXRs, RARs/RXRs and PPARs/RXRs can stimulate the release of GnRH in the hypothalamus to regulate seasonal estrus and proliferation of ovarian granulosa cells, remodeling of ovarian vascular tissue, and steroid production, which affect the animal estrous cycle conversion. This paper summarizes the role of RXRs and their dimers in the different reproductive activities related to seasonal estrus and estrous cycle of animals, and provides a reference for future research.

CLC Number:

S814.1

LI Huazhen, DI Ran, LIU Qiuyue, HU Wenping, WANG Xiangyu, MA Lin, LIU Wujun, CHU Mingxing. Research Progress on the Regulative Role of Retinoid X Receptors Gene in Animal Seasonal Estrus and Estrous Cycle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1525-1535.

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References

[1] GAMPE R J Jr,MONTANA V G,LAMBERT M H,et al.Structural basis for autorepression of retinoid X receptor by tetramer formation and the AF-2 helix[J].Genes Dev,2000,14(17):2229-2241.
[2] PINTO V M S,MINAKHINA S,QIU S Q,et al.Naturally occurring amino acids in helix 10 of the thyroid hormone receptor mediate isoform-specific TH gene regulation[J].Endocrinology,2017,158(9):3067-3078.
[3] WILLY P J,MANGELSDORF D J.Unique requirements for retinoid-dependent transcriptional activation by the orphan receptor LXR[J].Genes Dev,1997,11(3):289-298.
[4] 李华振,刘武军,刘秋月,等.甲状腺激素受体基因调控动物繁殖的研究进展[J].畜牧兽医学报,2019,50(2):243-252.
LI H Z,LIU W J,LIU Q Y,et al.Research progress on the regulative role of thyroid hormone receptor gene in animal reproduction[J].Acta Veterinaria et Zootechnica Sinica,2019,50(2):243-252.(in Chinese)
[5] SINGH B K,SINHA R A,OHBA K,et al.Role of thyroid hormone in hepatic gene regulation,chromatin remodeling,and autophagy[J].Mol Cell Endocrinol,2017,458:160-168.
[6] KERSTEN S,PAN L,CHAMBON P,et al.Role of ligand in retinoid signaling.9-cis-retinoic acid modulates the oligomeric state of the retinoid X receptor[J].Biochemistry,1995,34(42):13717-13721.
[7] HEYMAN R A,MANGELSDORF D J,DYCK J A,et al.9-cis retinoic acid is a high affinity ligand for the retinoid X receptor[J].Cell,1992,68(2):397-406.
[8] BOGACKA I,KURZYNSKA A,BOGACKI M,et al.Peroxisome proliferator-activated receptors in the regulation of female reproductive functions[J].Folia Histochem Cytobiol,2015,53(3):189-200.
[9] 周晓苏,王旭波,宋华玉,等.许氏平鲉三种促性腺激素释放激素基因的启动子克隆及生物信息学分析[J].中国海洋大学学报,2012,42(10):58-64.
ZHOU X S,WANG X B,SONG H Y,et al.Cloning and bioinformati analysis of the promoters from three gonadotropin-releasing hormone genes of sebastes schlegelii[J].Periodical of Ocean University of China,2012,42(10):58-64.(in Chinese)
[10] ROSS R A,LEON S,MADARA J C,et al.PACAP neurons in the ventral premammillary nucleus regulate reproductive function in the female mouse[J].eLife,2018,2(7):e35960.
[11] KLIEWER S A,UMESONO K,NOONAN D J,et al.Convergence of 9-cis retinoic acid and peroxisome proliferator signalling pathways through heterodimer formation of their receptors[J].Nature,1992,358(6389):771-774.
[12] FROMENT P,FABRE S,DUPONT J,et al.Expression and functional role of peroxisome proliferator-activated receptor-γ in ovarian folliculogenesis in the sheep[J].Biol Reprod,2003,69(5):1665-1674.
[13] VISWAKARMA N,JIA Y Z,BAI L,et al.Coactivators in PPAR-regulated gene expression[J].PPAR Res,2010,2010:250126.
[14] NESTLER J E,STOVALL D,AKHTER N,et al.Strategies for the use of insulin-sensitizing drugs to treat infertility in women with polycystic ovary syndrome[J].Fertil Steril,2002,77(2):209-215.
[15] KOMAR C M,BRAISSANT O,WAHLI W,et al.Expression and localization of PPARs in the rat ovary during follicular development and the periovulatory period[J].Endocrinology,2001,142(11):4831-4838.
[16] KOMAR C M,CURRY T J Jr.Localization and expression of messenger RNAs for the peroxisome proliferator-activated receptors in ovarian tissue from naturally cycling and pseudopregnant rats[J].Biol Reprod,2002,66(5):1531-1539.
[17] VIERA-VERA J,GARCIA-ARRARAS J E.Molecular characterization and gene expression patterns of retinoid receptors,in normal and regenerating tissues of the sea cucumber,Holothuria glaberrima[J].Gene,2018,654:23-35.
[18] SWIFT C B,HAYS J L,PETTY W J.Distinct functions of retinoic acid receptor beta isoforms:implications for targeted therapy[J].Endocr Metab Immune Disord Drug Targets,2008,8(1):47-50.
[19] XU X C.Tumor-suppressive activity of retinoic acid receptor-β in cancer[J].Cancer Lett,2007,253(1):14-24.
[20] TANG D,FAKIOLA M,SYN G,et al.Arylsulphatase A pseudodeficiency (ARSA-PD),hypertension and chronic renal disease in Aboriginal Australians[J].Sci Rep,2018,8(1):10912.
[21] MIZUNUMA H,LIU X W,ANDOH K,et al.Activin from secondary follicles causes small preantral follicles to remain dormant at the resting stage[J].Endocrinology,1999,140(1):37-42.
[22] ANTOSIK P,KEMPISTY B,JACKOWSKA M,et al.Differential expression of genes encoding EGF,IGF-I,TGFβ1,TGFβ2 and TGFβ3 in porcine endometrium during estrus cycle at different ages[J].Med Weter,2010,66(9):618-621.
[23] LU R,ITO J,IWAMOTO N,et al.FGF-1 induces expression of LXRα and production of 25-hydroxycholesterol to upregulate the apoE gene in rat astrocytes[J].J Lipid Res,2009,50(6):1156-1164.
[24] WAN X J,WANG S B,XU J R,et al.Dietary protein-induced hepatic IGF-1 secretion mediated by PPARγ activation[J].PLoS One,2017,12(3):e173174.
[25] ARMSTRONG D G,WEBB R.Ovarian follicular dominance:the role of intraovarian growth factors and novel proteins[J].Rev Reprod,1997,2(3):139-146.
[26] 白爱红,付秀虹,李荣香,等.多囊卵巢综合征患者血清miR-206和IGF-1的表达水平及临床意义[J].中国妇幼保健,2019,34(1):139-142.
BAI A H,FU X H,LI R X,et al.Expression levels of serum miR-206 and IGF-1 in patients with polycystic ovary syndrome and its clinical significance[J].Maternal and Child Health Care of China,2019,34(1):139-142.(in Chinese)
[27] NICKKHO-AMIRY M,MCVEY R,HOLLAND C.Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma[J].Mol Cancer Res,2012,10(3):441-453.
[28] JAGARLAMUDI K,LIU L,ADHIKARI D,et al.Oocyte-specific deletion of Pten in mice reveals a stage-specific function of PTEN/PI3K signaling in oocytes in controlling follicular activation[J].PLoS One,2009,4(7):e6186.
[29] DE VRIES W N,BINNS L T,FANCHER K S,et al.Expression of Cre recombinase in mouse oocytes:a means to study maternal effect genes[J].Genesis,2000,26(2):110-112.
[30] KIM J,SATO M,LI Q,et al.Peroxisome proliferator-activated receptor γ is a target of progesterone regulation in the preovulatory follicles and controls ovulation in mice[J].Mol Cell Biol,2008,28(5):1770-1782.
[31] YUAN Y,YEH L K,LIU H S,et al.Targeted overexpression of TGF-α in the corneal epithelium of adult transgenic mice induces changes in anterior segment morphology and activates noncanonical Wnt signaling[J].Invest Ophthalmol Vis Sci,2013,54(3):1829-1837.
[32] KELLER H,GIVEL F,PERROUD M,et al.Signaling cross-talk between peroxisome proliferator-activated receptor/retinoid X receptor and estrogen receptor through estrogen response elements[J].Mol Endocrinol,1995,9(7):794-804.
[33] LEE S J,YANG E K,KIM S G.Peroxisome proliferator-activated receptor-γ and retinoic acid X receptor α represses the TGFβ1 gene via PTEN-mediated p70 ribosomal S6 kinase-1 inhibition:role for Zf9 dephosphorylation[J].Mol Pharmacol, 2006, 70(1):415-425.
[34] PARBORELL F,ABRAMOVICH D,IRUSTA G,et al. Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway[J].Mol Cell Endocrinol,2011,343(1-2):79-87.
[35] BAKER J,HARDY M P,ZHOU J,et al.Effects of an Igf1 gene null mutation on mouse reproduction[J].Mol Endocrinol, 1996,10(7):903-918.
[36] FROMENT P,GIZARD F,DEFEVER D,et al.Peroxisome proliferator-activated receptors in reproductive tissues:from gametogenesis to parturition[J].J Endocrinol,2006,189(2):199-209.
[37] BOGACKA I,BOGACKI M.The quantitative expression of peroxisome proliferator activated receptor (PPAR) genes in porcine endometrium through the estrous cycle and early pregnancy[J].J Physiol Pharmacol,2011,62(5):559-565.
[38] HE Y,WANG C L.Effects of testosterone on PPARγ and P450arom expression in polycystic ovary syndrome patients and related mechanisms[J].Eur Rev Med Pharmacol Sci,2018,22(6):1549-1553.
[39] IUORNO M J,NESTLER J E.Insulin-lowering drugs in polycystic ovary syndrome[J].Obstetr Gyneco Clin North Amer,2001, 28(1):153-164.
[40] MU Y M,YANASE T,NISHI Y,et al.Combined treatment with specific ligands for PPARγ:RXR nuclear receptor system markedly inhibits the expression of cytochrome P450arom in human granulosa cancer cells[J].Mol Cell Endocrinol, 2001, 181(1-2):239-248.
[41] CUI Y Z,MIYOSHI K,CLAUDIO E,et al.Loss of the peroxisome proliferation-activated receptor gamma (PPARγ) does not affect mammary development and propensity for tumor formation but leads to reduced fertility[J].J Biol Chem,2002, 277(20):17830-17835.
[42] MINGE C E,RYAN N K,VAN DER HOEK K H,et al.Troglitazone regulates peroxisome proliferator-activated receptors and inducible nitric oxide synthase in murine ovarian macrophages[J].Biol Reprod,2006,74(1):153-160.
[43] LOVEKAMP-SWAN T,CHAFFIN C L.The peroxisome proliferator-activated receptor γ ligand troglitazone induces apoptosis and p53 in rat granulosa cells[J].Mol Cell Endocrinol,2005,233(1-2):15-24.
[44] TINFO N,KOMAR C.Potential role for peroxisome proliferator-activated receptor γ in regulating luteal lifespan in the rat[J]. Reproduction, 2007,133(1):187-196.
[45] HIROMORI Y,YUI H,NISHIKAWA J I,et al.Organotin compounds cause structure-dependent induction of progesterone in human choriocarcinoma Jar cells[J].J Steroid Biochem Mol Biol,2016,155:190-198.
[46] LEBOVIC D I,KIR M,CASEY C L.Peroxisome proliferator-activated receptor-γ induces regression of endometrial explants in a rat model of endometriosis[J].Fertil Steril,2004,82(3):1008-1013.
[47] KURZYNSKA A,CHOJNOWSKA K,BOGACKI M,et al.PPAR ligand association with prostaglandin F₂α and E₂ synthesis in the pig corpus luteum-an in vitro study[J].Anim Reprod Sci,2016,172:157-163.
[48] DARNERUD P O,RISBERG S.Tissue localisation of tetra- and pentabromodiphenyl ether congeners (BDE-47,-85 and -99) in perinatal and adult C57BL mice[J].Chemosphere,2006,62(3):485-493.
[49] DUPONT J,REVERCHON M,CLOIX L,et al.Involvement of adipokines,AMPK,PI3K and the PPAR signaling pathways in ovarian follicle development and cancer[J].Int J Dev Biol,2012,56(10-12):959-967.
[50] ZHANG H,LI Q L,LIN H Y,et al.Role of PPARγ and its gonadotrophic regulation in rat ovarian granulosa cells in vitro[J]. Neuro Endocrinol Lett,2007,28(3):289-294.
[51] CAO X H,LIU W,LIN F,et al.Retinoid X receptor regulates Nur77/thyroid hormone receptor 3-dependent apoptosis by modulating its nuclear export and mitochondrial targeting[J].Mol Cell Biol,2004,24(22):9705-9725.
[52] BONOFIGLIO D,GABRIELE S,AQUILA S,et al.Peroxisome proliferator-activated receptor gamma activates fas ligand gene promoter inducing apoptosis in human breast cancer cells[J].Breast Cancer Res Treat,2009,113(3):423-434.
[53] BELANI M,DEO A,SHAH P,et al.Differential insulin and steroidogenic signaling in insulin resistant and non-insulin resistant human luteinized granulosa cells-A study in PCOS patients[J].J Steroid Biochem Mol Biol,2018,178:283-292.
[54] HYUN S,KIM M S,SONG Y S,et al.Peroxisome proliferator-activated receptor-gamma agonist 4-O-methylhonokiol induces apoptosis by triggering the intrinsic apoptosis pathway and inhibiting the PI3K/Akt survival pathway in SiHa human cervical cancer cells[J].J Microbiol Biotechnol,2015,25(3):334-342.
[55] SZANTO A,NARKAR V,SHEN Q,et al.Retinoid X receptors:X-ploring their (patho)physiological functions[J].Cell Death Differ, 2004,11(2):S126-S143.
[56] HUANG Y S,CHANG C W,CHEN Y M,et al.Investigating expression profiles of VEGF-Flk,and Angpt1 during development of gas glands in Japanese eel (Anguilla japonica)[J].Comp Biochem Physiol A:Mol Integr Physiol,2010,155(3):350-360.
[57] IKEGAMI K,YOSHIMURA T.Comparative analysis reveals the underlying mechanism of vertebrate seasonal reproduction[J]. Gen Comp Endocrinol,2016,227:64-68.
[58] KAŠŠÁK F,HÁNA V,SAUDEK V,et al.Novel mutation (T273R) in thyroid hormone receptor β gene provides further insight into cryptic negative regulation by thyroid hormone[J].Folia Biol (Praha),2017,63(2):60-66.
[59] XIONG J J,KARSCH F J,LEHMAN M N.Evidence for seasonal plasticity in the gonadotropin-releasing hormone (GnRH) system of the ewe:changes in synaptic inputs onto GnRH neurons[J].Endocrinology,1997,138(3):1240-1250.
[60] ANDERSON G M,CONNORS J M,HARDY S L,et al.Thyroid hormones mediate steroid-independent seasonal changes in luteinizing hormone pulsatility in the ewe[J].Biol Reprod,2002,66(3):701-706.
[61] BURGOS-TRINIDAD M,KOENIG R J.Dominant negative activity of thyroid hormone receptor variant α2 and interaction with nuclear corepressors[J].Mol Cell Endocrinol,1999,149(1-2):107-114.
[62] DUFFY D J,KRSTIC A,HALASZ M,et al.Retinoic acid and TGF-β signalling cooperate to overcome MYCN-induced retinoid resistance[J].Genome Med,2017,9(1):15.
[63] SHIUE Y L,CHEN L R,TSAI C J,et al.Emerging roles of peroxisome proliferator-activated receptors in the pituitary gland in female reproduction[J].Biomark Genom Med,2013,5(1-2):1-11.
[64] 于嘉瑞,王翔宇,郭晓飞,等.LEPR及LEP基因表达量对FecB不同基因型小尾寒羊产羔数的影响[J].畜牧兽医学报,2018,49(5):954-961.
YU J R,WANG X Y,GUO X F,et al.Effects of expression of LEPR and LEP genes on litter size of Small Tail Han sheep with different FecB genotypes[J].Acta Veterinaria et zootechnica Sinica,2018,49(5):954-961.(in Chinese)
[65] SHIMIZU M,SAKAI H,SHIRAKAMI Y,et al.Acyclic retinoid inhibits diethylnitrosamine-induced liver tumorigenesis in obese and diabetic C57BLKS/J- + Lepr^db/+Lepr^db mice[J].Cancer Prev Res (Phila),2011,4(1):128-136.
[66] WANICHKUL T,HAN S W,HUANG R P,et al.Cytokine regulation by peroxisome proliferator-activated receptor gamma in human endometrial cells[J].Fertil Steril,2003,79(Suppl 1):763-769.
[67] RUEBNER M,LANGBEIN M,STRISSEL P L,et al.Regulation of the human endogenous retroviral Syncytin-1 and cell-cell fusion by the nuclear hormone receptors PPARγ/RXRα in placentogenesis[J].J Cell Biochem,2012,113(7):2383-2396.
[68] TAILOR S P,JAIN L S,GUPTA H K,et al.Oestrus and conception rates in buffaloes under village conditions[J].Indian J Anim Sci,1990,60(8):1020-1021.
[69] SARRUF D A,YU F,NGUYEN H T,et al.Expression of peroxisome proliferator-activated receptor-γ in key neuronal subsets regulating glucose metabolism and energy homeostasis[J].Endocrinology,2009,150(2):707-712.
[70] SHARMA S,SHARMA P M,MISTRY D S,et al.PPARG regulates gonadotropin-releasing hormone signaling in LbetaT2 cells in vitro and pituitary gonadotroph function in vivo in mice[J].Biol Reprod,2011,84(3):466-475.
[71] CEDERBERG R A,SMITH J E,MCDONALD E A,et al.Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region,two proximal GSEs and a retinoid X receptor binding site[J].Reprod Biol Endocrinol,2015,13:45.
[72] HOO R L C,CHAN K Y Y,LEUNG F K Y,et al.Involvement of NF-κB subunit p65 and retinoic acid receptors,RARα and RXRα, in transcriptional regulation of the human GnRH Ⅱ gene[J].FEBS J,2007,274(11):2695-2706.
[73] CHO S,CHO H,GEUM D,et al.Retinoic acid regulates gonadotropin-releasing hormone (GnRH) release and gene expression in the rat hypothalamic fragments and GT1-1 neuronal cells in vitro[J].Mol Brain Res,1998,54(1):74-84.
[74] CHO S,CHUNG J,HAN J,et al.9-cis-Retinoic acid represses transcription of the gonadotropin-releasing hormone (GnRH) gene via proximal promoter region that is distinct from all-trans-retinoic acid response element[J].Mol Brain Res,2001, 87(2):214-222.

Research Progress on the Regulative Role of Retinoid X Receptors Gene in Animal Seasonal Estrus and Estrous Cycle

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